𝔖 Bobbio Scriptorium
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Human antibody response to Toscana virus glycoproteins expressed by recombinant baculovirus

✍ Scribed by Paola Di Bonito; Simona Bosco; Stefania Mochi; Luisa Accardi; Maria G. Ciufolini; Loredana Nicoletti; Colomba Giorgi

Book ID
102382508
Publisher
John Wiley and Sons
Year
2002
Tongue
English
Weight
136 KB
Volume
68
Category
Article
ISSN
0146-6615

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✦ Synopsis

Abstract

The arthropod‐borne Toscana virus has been associated with acute neurological disease in humans. In this study, the viral envelope glycoproteins were expressed in soluble form in a baculovirus system. The recombinant sGN and sGC proteins were used as viral antigens in a Western blot assay to analyze the specific immune response in sera from patients with recognized virus‐associated aseptic meningitis. The anti‐glycoprotein and the anti‐nucleoprotein N IgG responses were compared by an immunoassay based on the recombinant proteins. In this system, all the sera showed a high reactivity to the N protein, but they differed in the response to the glycoproteins. J. Med. Virol. 68:615–619, 2002. © 2002 Wiley‐Liss, Inc.

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