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Human acylphosphatase cannot replace phosphoglycerate kinase in Saccharomyces cerevisiae

✍ Scribed by Pimvan Hoek; Alessandra Modesti; Giampietro Ramponi; Peter Kötter; Johannes P. van Dijken; Jack T. Pronk


Book ID
110309011
Publisher
Springer Netherlands
Year
2001
Tongue
English
Weight
169 KB
Volume
80
Category
Article
ISSN
0003-6072

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This study demonstrates that the isolated regulatory (R) domain (amino acids 1 -270) of human protein kinase C a (PKCa) is a potent inhibitor of PKCP-I activity in a yeast expression system. The PKCa R domain fused to glutathione-S-transferase competitively inhibited the activity of yeast-expressed