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HuC:Kaede, a useful tool to label neural morphologies in networks in vivo

✍ Scribed by Tomomi Sato; Mikako Takahoko; Hitoshi Okamoto

Publisher: John Wiley and Sons
Year: 2006
Tongue: English
Weight: 847 KB
Volume: 44
Category: Article
ISSN: 1526-954X
DOI: 10.1002/gene.20196

No coin nor oath required. For personal study only.

✦ Synopsis

A simple and efficient procedure for labeling neurons is a prerequisite for investigating the development of neural networks in zebrafish. To label neurons we used Kaede, a fluorescent protein with a photoconversion property allowing conversion from green to red fluorescence following irradiation with UV or violet light. We established a zebrafish stable transgenic line, Tg(HuC:Kaede), expressing Kaede in neurons under the control of the HuC promoter. This transgenic line was used to label a small number of neurons in the trigeminal ganglion. Also, using embryos injected with the transgene, we labeled peripheral axon arbors of a Rohon-Beard neuron at 4 days postfertilization and observed the dendrite development of a tectal neuron for 3 days. These data indicate that Kaede is a useful tool to selectively label neural networks in zebrafish. genesis 44: 136-142, 2006. V

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