HPTLC fingerprint identification of commercial ginseng drugs - reinvestigation of HPTLC of ginsenosides
✍ Scribed by Peishan, Xie ;Yuzhen, Yan
- Publisher
- John Wiley and Sons
- Year
- 1987
- Tongue
- English
- Weight
- 503 KB
- Volume
- 10
- Category
- Article
- ISSN
- 0935-6304
No coin nor oath required. For personal study only.
✦ Synopsis
The optimized HPTLC of ginsenosides reported in this paper shows that the recommended solvent system has a higher resolution, greater spot capacity, and better reproducibility by comparison with the established ones in the previous literature. Detection and scanning in the fluorescence mode after visualization with 5 % sulfuric acidlEtOH by the dipping technique improved and enhanced the sensitivity by a factor of nine compared to the commonly used absorbance mode.
Sample pretreatment by an adsorption clean-up step on a small alumina column followed by 1 -butanol extraction instead of only a butanol-extraction step made the chromatogram clearer, caused less background contamination, and reduced the tailing of some ginsenosides spots.
The 'peak grouping' method was effectively used in HPTLC fin- gerprint identification of various commercial ginseng medicines and the chromatograms of roots of ginseng (white and red; Panax ginseng), American ginseng (P.quinquifolium), and sanchi (P. notoginseng) obtained under recommended condition are perfectly recognizable.
MS