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HPTLC-densitometric method of determination of oleanolic acid in the Lamii albi flos

✍ Scribed by Magdalena Wójciak-Kosior; Tadeusz Krzaczek; Grazyna Matysik; Agnieszka Skalska

Publisher
John Wiley and Sons
Year
2005
Tongue
English
Weight
434 KB
Volume
28
Category
Article
ISSN
1615-9306

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✦ Synopsis

Abstract

HPTLC combined with densitometric method was used to determine the content of oleanolic acid in acetone extract from the Lamii albi flos L. The plant extract was separated on Si 60 HPTLC plates and determined with the use of two densitometric methods: measurement of fluorescence and absorbance. The content of oleanolic acid was statistically calculated. In a measurement of absorbance in 1 g of the dry Lamii albi flos L. 1458 μg of oleanolic acid was determined (0.147%). In a fluorescence method in 1 g of the plant material 1516 μg of oleanolic acid was determined (0.149%).

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✍ Milind Bagul; H. Srinivasa; Harish Padh; M. Rajani 📂 Article 📅 2005 🏛 John Wiley and Sons 🌐 English ⚖ 660 KB

Gallic acid and ellagic acid are two widely occurring phenolic compounds of plant origin, to which many biological activities including anticancer and antiviral activity have been attributed. A simple HPTLC method has been developed for the simultaneous quantification of gallic acid and ellagic acid