HPTLC coupled with bioluminescence and mass spectrometry for bioactivity-based analysis of secondary metabolites in marine sponges

Use of ultra-performance liquid chromato

Use of ultra-performance liquid chromatography/time-of-flight mass spectrometry with nozzle-skimmer fragmentation for comprehensive quantitative analysis of secondary metabolites in Arabidopsis thaliana

✍ Takanori Sugimoto; Takeshi Bamba; Yoshihiro Izumi; Hironari Nomura; Takashi Shii 📂 Article 📅 2011 🏛 John Wiley and Sons 🌐 English ⚖ 132 KB

## Abstract This study sought to develop techniques for LC/MS‐based metabolomics and to verify that an MS/MS spectral tag (MS2T) could be used in practical secondary metabolite profiling. The retention time (RT), precursor ions, and fragment ions generated by nozzle‐skimmer fragmentation were deter