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HPLC method for amino acids profile in biological fluids and inborn metabolic disorders of aminoacidopathies

โœ Scribed by S. V. Suresh Babu; M. M. Shareef; A. Pavan Kumar Shetty; K. Taranath Shetty


Publisher
Association of Clinical Biochemistry of India
Year
2002
Tongue
English
Weight
914 KB
Volume
17
Category
Article
ISSN
0970-1915

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HPLC method for amino acids profile in b
โœ S. V. Suresh Babu; M. M. Shareef; A. Pavan Kumar Shetty; K. Taranath Shetty ๐Ÿ“‚ Article ๐Ÿ“… 2002 ๐Ÿ› Association of Clinical Biochemistry of India ๐ŸŒ English โš– 914 KB

Quantification of total and individual amino acids in biological fluids such as plasma, urine and cerebrospinal fluid has an important diagnostic implication in laboratory medicine. The present paper describes protocols for the assay of total amino acids by modified method based on dinitrophenyl and

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A method is described for obtaining plasma ultrafiltrates from which the concentrations of all amino acids, including tryptophan and ammonia, are obtained. A split-stream methodology is described for obtaining, in addition to the concentrations, the radioactivities of amino acids, glucose, and plasm