Quantification of total and individual amino acids in biological fluids such as plasma, urine and cerebrospinal fluid has an important diagnostic implication in laboratory medicine. The present paper describes protocols for the assay of total amino acids by modified method based on dinitrophenyl and
โฆ LIBER โฆ
HPLC method for amino acids profile in biological fluids and inborn metabolic disorders of aminoacidopathies
โ Scribed by S. V. Suresh Babu; M. M. Shareef; A. Pavan Kumar Shetty; K. Taranath Shetty
- Publisher
- Association of Clinical Biochemistry of India
- Year
- 2002
- Tongue
- English
- Weight
- 914 KB
- Volume
- 17
- Category
- Article
- ISSN
- 0970-1915
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A method is described for obtaining plasma ultrafiltrates from which the concentrations of all amino acids, including tryptophan and ammonia, are obtained. A split-stream methodology is described for obtaining, in addition to the concentrations, the radioactivities of amino acids, glucose, and plasm