HPLC for Pharmaceutical Scientists (Kazakevich/HPLC for Pharmaceutical Scientists) || Size-Exclusion Chromatography

✍ Scribed by Kazakevich, Yuri; LoBrutto, Rosario

• Publisher: John Wiley & Sons, Inc.
• Year: 2006
• Tongue: English
• Weight: 450 KB
• Edition: 1
• Category: Article
• ISBN: 0471681628
• DOI: 10.1002/9780470087954.ch6

✦ Synopsis

Size-exclusion chromatography (SEC) separates polymer molecules and biomolecules based on differences in their molecular size. The separation process in simplified form is based on the ability of sample molecules to penetrate inside the pores of packing material and is dependent on the relative size of analyte molecules and the respective pore size of the absorbent. The process also relies on the absence of any interactions with the packing material surface. Two types of SEC are usually distinguished:

1. Gel permeation chromatography (GPC)-separation of synthetic (organic-soluble) polymers. GPC is a powerful technique for polymer characterization using primarily organic solvents. 2. Gel filtration chromatography (GFC)-separation of water-soluble biopolymers. GFC uses primarily aqueous solvents (typically for aqueous soluble polymers, proteins, etc.).

Physical and chemical properties of polymers are dependent on their molecular weight and molecular weight distribution.The separation principle in SEC is based on the forced transport of the polymer molecules through the porous stationary-phase media under the conditions of suppressed interactions of the