HPLC enantiomeric resolution of novel aromatase inhibitors on cellulose- and amylose-based chiral stationary phases under reversed phase mode

✍ Scribed by Hassan Y. Aboul-Enein; Imran Ali; Gerald Gübitz; Claire Simons; Paul J. Nicholls

• Publisher: John Wiley and Sons
• Year: 2000
• Tongue: English
• Weight: 149 KB
• Volume: 12
• Category: Article
• ISSN: 0899-0042
• DOI: 10.1002/1520-636x(2000)12:10<727::aid-chir5>3.0.co;2-t

✦ Synopsis

The chiral resolution of seven aromatase inhibitors (four triazole derivatives (Ia, Ib, Ic, and Id) and three tetrazole derivatives (IIa, IIb, and IIc)) was achieved on Chiralcel OJ-R [cellulose tris (4-methyl benzoate)], Chiralcel OD-RH [cellulose tris (3,5-dimethylphenyl carbamate)], and Chiralpak AD-RH [amylose tris (3,5dimethylphenyl carbamate)] chiral stationary phases. The mobile phases used were A: 2-PrOH-MeCN (90:10, v/v); B: 2-PrOH-MeCN (50:50, v/v); C: MeCN-H 2 O (50:50, v/v); D: MeCN-H 2 O (80:20, v/v); and E: MeCN-H 2 O (95:05, v/v). The flow rate was 0.5 mL/min for all the mobile phases. The resolution capability of these chiral stationary phases were in the order Chiralpak AD-RH > Chiralcel OD-RH > Chiralcel OJ-R. The values of ␣ and Rs of the resolved enantiomers of the aromatase inhibitors varied from 1.