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HPLC assay of underivatized free amino acids with column switching and evaporative light-scattering detection

✍ Scribed by das Neves, Higuinaldo J. Chaves ;Morais, Zilda Braga

Publisher
John Wiley and Sons
Year
1997
Tongue
English
Weight
412 KB
Volume
20
Category
Article
ISSN
0935-6304

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✦ Synopsis

Abstract

In this communication the authors report the HPLC SEPARATION OF 20 amino acids without derivatization. Separation was achieved by column swithing using a C‐8 reversed‐phase column and a cationic ion‐exchange column. Detection of the underivatized amino acids was accomplished by means of an evaporative laser light‐scattering (ELS) detector. The influence of the temperature of the diffusion tube and nebuolzation gas pressure on detector response is reported. The relationship of peak areas vs. mass show a good log‐log linearity within a 1:10 range. Detechtion limit is in the order of 200 pmol for most amino acids under the conditions used.

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Evaporative light-scattering detection c
Evaporative light-scattering detection coupled to microcolumn liquid chromatography for the analysis of underivatized amino acids: Sensitivity, linearity of response and comparisons with UV absorbance detection
✍ Z. Cobb; P. N. Shaw; L. L. Lloyd; N. Wrench; D. A. Barrett 📂 Article 📅 2001 🏛 John Wiley and Sons 🌐 English ⚖ 135 KB

## Abstract Microcolumn chromatography (1.0 mm to 0.3 mm column i.d.) of underivatized amino acids coupled to evaporative light‐scattering (ELS) detection demonstrated good baseline stability using perfluorinated carboxylic acid ion‐pairing agents of varying alkyl chain lengths (C~2~–C~8~) and conc