✦ LIBER ✦

HPLC analysis of tear proteins in giant papillary conjunctivitis

✍ Scribed by M. S. A. Suttorp-Schulten; L. Luyendijk; J. H. C. Kok; A. Kijlstra

Publisher: Springer-Verlag
Year: 1989
Tongue: English
Weight: 227 KB
Volume: 72
Category: Article
ISSN: 0012-4486
DOI: 10.1007/bf00153490

No coin nor oath required. For personal study only.

✦ Synopsis

Giant papillary conjunctivitis (GPC) is a well defined entity seen in patients with deposits on their lenses. High performance liquid chromatography (HPLC) tear protein analysis was performed on 17 GPC patients and compared with healthy controls with and without contact lenses. The IgA levels are somewhat lower in GPC patients as compared with healthy controls not wearing lenses (P < 0.05). A similar decrease was also seen in the control group wearing contact lenses. We were not able to identify a specific HPLC tear protein profile in GPC patients as compared with controls. The HPLC profile therefore gives no explanation for the lens deposits and is not suitable as a diagnostic or prognostic tool.

📜 SIMILAR VOLUMES

M cells are involved in pathogenesis of

M cells are involved in pathogenesis of human contact lens-associated giant papillary conjunctivitis

✍ Xingwu Zhong; Hongshan Liu; Aijun Pu; Xuefeng Xia; Xiaodong Zhou 📂 Article 📅 2007 🏛 Springer 🌐 English ⚖ 815 KB

Analysis of human tear protein profiles

Analysis of human tear protein profiles using high performance liquid chromatography (HPLC)

✍ R. J. Boukes; A. Boonstra; A. C. Breebaart; D. Reits; E. Glasius; L. Luyendyk; A 📂 Article 📅 1987 🏛 Springer-Verlag 🌐 English ⚖ 431 KB

Using high performance liquid chromatography (HPLC) the tear protein profiles were measured in controls, patients with Sj6gren's disease, questionable dry eye (idiopathic dry eye), idiopathic chronic conjunctivitis and the corneal melting syndrome. Qualitative comparison of the protein profiles of

N-acetyl-aspartyl glutamic acid (NAAGA)

N-acetyl-aspartyl glutamic acid (NAAGA) topical eyedrops in the treatment of Giant Papillary Conjunctivitis (GPC)

✍ F. Meijer; K. Pogany; J. H. C Kok; A. Kijlstra 📂 Article 📅 1993 🏛 Springer-Verlag 🌐 English ⚖ 379 KB

HPLC analysis of free amino acids and am

HPLC analysis of free amino acids and amino acids of total proteins in cultured cells: An application to the study of rat sertoli cell protein metabolism

✍ S. Palmero; M. de Marchis; M. Prati; E. Fugassa 📂 Article 📅 1992 🏛 Elsevier Science 🌐 English ⚖ 701 KB

A simple, rapid and, sensitive HPLC method, coupled with fluorometric detection, has been worked out and employed to determine the intracellular free amino acid concentrations and the amino acid composition of total proteins in rat Sertoli cell primary culture. Sertoli cells were isolated enzymatica

Single eye analysis and contralateral ey

Single eye analysis and contralateral eye comparison of tear proteins in normal and dry eye model rabbits by MALDI-ToF mass spectrometry using wax-coated target plates

✍ Bryan M. Ham; Jean T. Jacob; Richard B. Cole 📂 Article 📅 2007 🏛 Springer 🌐 English ⚖ 646 KB

Amino acid composition analysis of minut

Amino acid composition analysis of minute amounts of cysteine-containing proteins using 4-(Aminosulfonyl)-7-fluoro-2,1,3-benzoxadiazole and 4-fluoro-7-nitro-2,1,3-benzoxadiazole in combination with HPLC

✍ Toshimasa Toyo'oka; Hiroshi Miyano; Kazuhiro Imai 📂 Article 📅 1986 🏛 John Wiley and Sons 🌐 English ⚖ 554 KB

The simultaneous determination of amino acid composition including cysteine of egg albumin, a model protein containing a/s cysteine residue, is reported. All the thiol groups of the cysteine residue@) of egg albumin were labelled with 4-(aminosulfonyl)-7-fluoro-2,1,3-benzoxadiazole, a fluorogenic re