How to predict product yield in kinetically controlled enzymatic peptide synthesis

✍ Scribed by Mikhail Yu. Gololobov; Elena V. Kozlova; Ivan L. Borisov; Ute Schellenberger; Volker Schellenberger; Hans-Dieter Jakubke

• Publisher: John Wiley and Sons
• Year: 1992
• Tongue: English
• Weight: 455 KB
• Volume: 40
• Category: Article
• ISSN: 0006-3592
• DOI: 10.1002/bit.260400313

✦ Synopsis

Abstract

The published theory of kinetically controlled enzymatic peptide synthesis needed experimental verification. We carried out the measurement of the peptide yield and estimation of the key parameters α and β for papain‐catalyzed synthesis of Mal‐L‐Phe‐L‐Ala‐LLeuNH~2~ from Mal‐L‐Phe‐L‐AlaOMe and L‐LeuNH~2~. The experimental results demonstrate that this theory adequately describes the real process. © 1992 John Wiley & Sons, Inc.