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How Do Chemical Denaturants Affect the Mechanical Folding and Unfolding of Proteins?

✍ Scribed by Yi Cao; Hongbin Li


Book ID
116663625
Publisher
Elsevier Science
Year
2008
Tongue
English
Weight
639 KB
Volume
375
Category
Article
ISSN
0022-2836

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It has been shown that human stefin B exhibits molten globule intermediates when denatured by acid or GuHCl. In the presence of TFE, it transforms into a highly helical state. In our first study on its folding mechanism (Z Λ‡erovnik et al., Proteins 32:296-303), the kinetics measured by circular dich