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Host specificity of DNA produced byEscherichia coli

✍ Scribed by Grete Kellenberger; Neville Symonds; Werner Arber


Publisher
Springer
Year
1966
Tongue
English
Weight
644 KB
Volume
98
Category
Article
ISSN
1617-4615

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✍ V. B. Yadwad; K. Meghji; O. P. Ward πŸ“‚ Article πŸ“… 1993 πŸ› Springer-Verlag 🌐 English βš– 542 KB

Human recombinant EGF, secreted into the extracellular medium by E. c&i cells, was purified by a combination of solid phase extraction and HPLC. Using these techniques, the peptide was purified 122-fold, with a recovery of greater than 75%. The purified hEGF manifested no contaminating protein bands