Horseradish-root-modified carbon paste bioelectrode

✍ Scribed by Joseph Wang; Meng Shan Lin

• Book ID: 102180311
• Publisher: John Wiley and Sons
• Year: 1989
• Tongue: English
• Weight: 367 KB
• Volume: 1
• Category: Article
• ISSN: 1040-0397
• DOI: 10.1002/elan.1140010107

✦ Synopsis

The incorporation of horseradish roots into the carbon paste matrix results in an effective bioelectrode for sensing hydrogen peroxide. The response is based on the hydrogen peroxidase activity in the tissue. Because of the intimate contact between the biocatalytic and sensing sites, a very Past response is observed, as desired for practical biosensing applications. The behavior of the new bioelectrode is characterized using a flow injection system. Response time (to5%) as low as 11 s has been determined.

The limit of detection is 3 X lo-' M (1 ng), the relative standard deviation is 1.4%, and the response is linear up to 1.2 X M. Oxidizable biological compounds do not interfere in the determination of hydrogen peroxide. The response is characterized also with respect to paste composition, flow rate, applied potential, reducing-agent concentration, injection volume, and other variables. Rapid and sensitive flow-injection measurements of glucose are illustrated. The tissue-modified electrode possesses many of the features desirable for use as an amperometric sensor for oxidase substrates.