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Horseradish peroxidase catalyzed degradation of industrially important dyes

โœ Scribed by Anirban Bhunia; Susheel Durani; Pramod P. Wangikar

Publisher
John Wiley and Sons
Year
2001
Tongue
English
Weight
117 KB
Volume
72
Category
Article
ISSN
0006-3592

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โœฆ Synopsis

Horseradish peroxidase (HRP) is known to degrade certain recalcitrant organic compounds such as phenol and substituted phenols. Here, for the first time we have shown HRP to be effective in degrading and precipitating industrially important azo dyes. For Remazol blue, the enzyme activity was found to be far better at pH 2.5 than at neutral pH. In addition, Remazol blue acts as a strong competitive inhibitor of HRP at neutral pH. Horseradish peroxidase shows broad substrate specificity toward a variety of azo dyes. Kinetic constants (K m app and V max app ) for two different dyes have been determined. In addition to providing a systematic analysis of the potential of HRP in degradation of dyes, this study opens up a new area on exploration of commercial dyes as inhibitors of enzymes. 2001

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