β Scribed by Michael J. Wheeler PhD, FRCPath (auth.), Michael J. Wheeler, J. S. Morley Hutchinson (eds.)
No coin nor oath required. For personal study only.
Although automated systems can measure all common hormones in human blood, they are expensive and often incapable of measuring hormones in urine, saliva, and animals. In Hormone Assays in Biological Fluids, expert researchers who have developed and applied significant new assays describe in step-by-step detail readily reproducible methods for measuring a broad variety of hormones, related peptides, and synthetic steroids in various biological fluids. The hormones measured range from glucocorticoids in biological fluids, urinary steroids, aldosterone in blood, and plasma renin activity, to gut hormones in plasma, melatonin, prolactin, 6-sulfatoxymelatonin, and androgens in blood, saliva, and hair. The emphasis is on noncommercial assays so that investigators can develop novel methods suited to their special needs. Commercial assays are also described for comparative purposes. Tutorials on radioimmunoassay, gas chromatography-mass spectrometry, high-performance liquid chromatography, and polymerase chain reaction techniques help readers choose the best methods for their purpose. The protocols follow the successful Methods in Molecular Medicineβ’ series format, each offering step-by-step laboratory instructions, an introduction outlining the principles behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls.
Wide-ranging and highly practical, Hormone Assays in Biological Fluids illuminates the many different methods available for a broad range of hormone assays, enabling researchers to choose the optimal existing methods or develop novel assays for their specialized purposes.
Front Matter....Pages i-ix
Back Matter....Pages 1-23
....Pages 25-52
Biochemistry, general
π SIMILAR VOLUMES
<p><p>Hormone measurement is necessary for the diagnosis of a wide range of clinical conditions and is essential for monitoring the effectiveness of treatment. As the number of hormone requests in the clinical field rises exponentially, it has become imperative to create hormone assays accessible to
<p><span>Hormone measurement is necessary for the diagnosis of a wide range of clinical conditions and is essential for monitoring the effectiveness of treatment. As the number of hormone requests in the clinical field rises exponentially, it has become imperative to create hormone assays accessible
Expert researchers who have developed and applied significant new assays describe in step-by-step detail a variety of methods for measuring a broad variety of hormones, related peptides, and synthetic steroids in various biological fluids. The hormones measured range from glucocorticoids in biologic
