Homogeneous assay for real-time and simultaneous detection of thymidine kinase 1 and deoxycytidine kinase activities
✍ Scribed by Stålhandske, Per; Wang, Liya; Westberg, Sara; von Euler, Henrik; Groth, Erika; Gustafsson, Sven A.; Eriksson, Staffan; Lennerstrand, Johan
- Book ID
- 122053227
- Publisher
- Elsevier Science
- Year
- 2013
- Tongue
- English
- Weight
- 967 KB
- Volume
- 432
- Category
- Article
- ISSN
- 0003-2697
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We report a simple method that permits simultaneous detection of multiple protein kinase activities using postnuclear supernatant of \(\mathbf{v}-8\) src transformed NIH3T3 cells. A supernatant is incubated with activators of protein kinases and \(\left[\gamma-{ }^{32} \mathbf{P}\right] \mathrm{ATP}
## Abstract We previously isolated an acyclovir (ACV)‐resistant herpes simplex virus type 1 (HSV‐1), strain TAR, from a child with Wiskott‐Aldrich syndrome. An acyclovir‐sensitive HSV‐1, strain TAS, had been isolated from the same patient before the isolation of HSV‐1 TAR. The TK protein of ACV‐sen