Hollow-fibre affinity cell separation system for CD34+ cell enrichment

✍ Scribed by Robert E. Nordon; David N. Haylock; Leonie Gaudry; Klaus Schindhelm

• Book ID: 102651529
• Publisher: John Wiley and Sons
• Year: 1996
• Tongue: English
• Weight: 750 KB
• Volume: 24
• Category: Article
• ISSN: 0196-4763
• DOI: 10.1002/(sici)1097-0320(19960801)24:4<340::aid-cyto5>3.0.co;2-j

✦ Synopsis

28 min), a depleted fraction was collected at 5 dynedun' followed by washes at 10 and 25 dynedcm2. Antigen-positive cells were recovered after incubation with chymopapain. The device was tested by using peripheral blood mononuclear cells from seven patients who had received granulocyte colony-stimulating Eactor and chemotherapy. The average number of cells processed was 1.3 0.2 X 10' (+S.E.M.), and the preselection incidence of CD34+ cells was 1.6 f 0.6% (range 0.21-4.13%; n = 7). The enrichment purity was 94.4 * 3.1%, and 61 f 9% of input CD34+ cells were recovered in the enriched &action (n = 4). Enrichment resulted in a 3.3 f 0.1 log,, depletion of CD34-cells (n = 4). Hollow-fibre affinity cell separation has potential as a medium to large-scale cell enrichment technology. o 19% wuq-~iss, IIIC.