𝔖 Bobbio Scriptorium
✦   LIBER   ✦

HnRNP E2 is downregulated in human oral cancer cells and the overexpression of hnRNP E2 induces apoptosis

✍ Scribed by Paromita Roychoudhury; Ranjan Rashmi Paul; Rajdeep Chowdhury; Keya Chaudhuri

Publisher
John Wiley and Sons
Year
2007
Tongue
English
Weight
265 KB
Volume
46
Category
Article
ISSN
0899-1987

No coin nor oath required. For personal study only.

✦ Synopsis

Abstract

Human hnRNP genes have been reported to be involved in human malignancies and several hnRNPs are promising biomarkers of lung, head and neck, colon, breast, and pancreatic cancers. The present study investigated the clinicopathologic and biological significance of hnRNP E2 gene expression in oral cancer. Human hnRNP E2 was significantly downregulated in oral cancer tissues compared to normal one (P < 0.0001) as determined by quantitative real‐time reverse transcription PCR. The expression of hnRNP E2 is correlated with histology, being lower in moderate and poorly differentiated squamous cell carcinoma (SCC) compared to well‐differentiated SCC. Transient transfection of hnRNP E2 in cancerous cell lines resulted in reduced cell viability and increased apoptotic nuclei. Compared to control transfectants, cells with higher expression showed an increase in the number of apoptotic cells by annexin‐PI staining and an increase in caspase activity. The present study thus implicates downregulation of hnRNP E2 as a novel mechanism to enhance the resistance of cancer cells to apoptosis. © 2006 Wiley‐Liss, Inc.

📜 SIMILAR VOLUMES

miR-149* induces apoptosis by inhibiting
miR-149* induces apoptosis by inhibiting Akt1 and E2F1 in human cancer cells
✍ Ruey-Jen Lin; You-Chin Lin; Alice L. Yu 📂 Article 📅 2010 🏛 John Wiley and Sons 🌐 English ⚖ 345 KB

microRNAs (miRNAs) play vital roles in several biological processes, including apoptosis, by negatively regulating the expression of target genes. The molecular mechanisms of the key survival signal, Akt family, have been widely explored. However, it remains to be ascertained whether Akt1, the predo

Overexpression of bcl-2 regulates sodium
Overexpression of bcl-2 regulates sodium butyrate- and/or docetaxel-induced apoptosis in human bladder cancer cells both in vitro and in vivo
✍ Hideaki Miyake; Shoji Hara; Soichi Arakawa; Sadao Kamidono; Isao Hara 📂 Article 📅 2001 🏛 John Wiley and Sons 🌐 French ⚖ 188 KB 👁 1 views

Sodium butyrate (NaBt), a physiologically occurring shortchain fatty acid, induces differentiation as well as apoptosis in numerous cell types, and this induction is partially regulated by Bcl-2 expression. The objectives of our study were to characterize the in vitro effects of NaBt and/or docetaxe

The localization of hnRNP A2/B1 in nucle
The localization of hnRNP A2/B1 in nuclear matrix and the aberrant expression during the RA-induced differentiation of human neuroblastoma SK-N-SH cells
✍ Ying Liang; Song-Lin Shi; Qi-Fu Li; Lan-Ying Chen; Guang-Jun Jing; Guo-Wei Tan; 📂 Article 📅 2011 🏛 John Wiley and Sons 🌐 English ⚖ 432 KB 👁 2 views

Heterogeneous nuclear ribonucleoprotein (hnRNP) A2/B1 is involved in the synthesis of RNA. Its expression is up-regulated in many tumor cell lines. In this study, we investigated the distribution of hnRNP A2/B1 in the nuclear matrix, including its co-localization with expression products of related

The nucleocytoplasmic shuttling of E2F4
The nucleocytoplasmic shuttling of E2F4 is involved in the regulation of human intestinal epithelial cell proliferation and differentiation
✍ Claude Deschênes; Laetitia Alvarez; Marie-Ève Lizotte; Anne Vézina; Nathalie Riv 📂 Article 📅 2003 🏛 John Wiley and Sons 🌐 English ⚖ 486 KB 👁 1 views

## Abstract The specific mechanisms controlling the transition from proliferation to terminal differentiation in human intestinal epithelial cells (HIEC) remain largely undefined. Herein, we analyzed the expression and localization of Rb and E2F proteins in well‐established normal intestinal epithe