HLA-DQ RFLP variants of fiveHLA-DQw2-bearing major histocompatability complex extended haplotypes
✍ Scribed by Juan J. Yunis; Mark Wescott; Scarlet J. Lechin; Sharon M. Alosco; Zuheir Awdeh; Chester A. Alper; Edmond J. Yunis
- Book ID
- 104666058
- Publisher
- Springer-Verlag
- Year
- 1990
- Tongue
- English
- Weight
- 683 KB
- Volume
- 32
- Category
- Article
- ISSN
- 0093-7711
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✦ Synopsis
We have analyzed genomic DNA in a large number of independent examples of five HLA-DQw2-bearing extended haplotypes for their associated subtypes by restriction fragment length polymorphism (RFLP) using DRB, DQA, and DQB probes after Taq I and Pst I digestion and Southern blotting. In addition to three previously described HLA-DQw2 subtypes, DQw2a, DQw2b, and DQw2c, we observed a fourth subtype, HLA-DQw2d, characterized by 5.8 kilobase (kb) DRB/Taq I, 2.4, 2.3, and 1.8 kb DQB/Taq I, and 8.0 and 2.3 kb DQA/Pst I fragments. All 22 independent examples of the extended haplotype [HLA-B8, SC01, DR3] carried DQw2a and all 11 independent examples of [HLA-B18, F1C30, DR3] carried DQw2b. In addition, all independent examples (21 and 4, respectively) of two DR7-carrying extended haplotypes, [HLA-B44, FC31, DR7] and [HLA-Bw47, FC91,0,DR7], carried DQw2c and all independent examples of [HLA-Bw57, SC61, DR7] carried DQw2d. Our results show that the DNA in the DR/DQ region of extended haplotypes is relatively fixed and that different DQw2 subtypes characterize different DQw2-bearing extended haplotypes.