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HLA-DQ polymorphism analyzed by sequential restriction endonuclease DNA digestion

✍ Scribed by Massimo Trucco; Sharon Rosenshine; Isabella Cascino; René J. Duquesnoy


Book ID
104741341
Publisher
Springer-Verlag
Year
1986
Tongue
English
Weight
696 KB
Volume
24
Category
Article
ISSN
0093-7711

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✦ Synopsis


A direct, physical correspondence between certain Pst I-generated genomic DNA fragments and Taq I-generated fragments, revealed with HLA-DQ alpha or -DQ beta gene probes, has been demonstrated. As an immediate consequence, the nature of the DQ and DX hybridized genes contained in the fragments was established. Taq I-generated DQ allelic forms which associate with serologically defined DR1, DR2, and DRw6 specificities were also proven to be sensu stricto "splits" of the Pst I-generated allelic form associated with all three DR specificities.