HLA Class ii sequence polymorphisms and susceptibility to rheumatoid arthritis in greeks. the hla–drβ shared-epitope hypothesis accounts for the disease in only a minority of greek patients
✍ Scribed by Kyriaki A. Boki; Gabriel S. Panayi; Robert W. Vaughan; Alexandros A. Drosos; Haralampos M. Moutsopoulos; Jerry S. Lanchbury
- Publisher
- John Wiley and Sons
- Year
- 1992
- Tongue
- English
- Weight
- 633 KB
- Volume
- 35
- Category
- Article
- ISSN
- 0004-3591
No coin nor oath required. For personal study only.
✦ Synopsis
Objective. In Northern Europeans, rheumatoid arthritis (RA) is strongly associated with a relatively conserved pentapeptide sequence of HLA-DRP found notably in the HLA-DR4 subtypes Dw4 and Dw14 and in DRl. A previous serologic study of HLA class I1 polymorphism in a Greek population with RA failed to show significant associations with any antigen.
Methuh. We characterized HLA-DRB polymorphism in Greek patients with RA and in control subjects by restriction fragment length polymorphism analysis. Allelic DRB subtypes were examined by polymerase chain reaction amplification and oligonucleotide hybridization.
Resulls. DNA analysis in the RA patients showed that although individual HLA-DR allelic associations were weak, a relatively conserved HLA-DRf? motif was significantly associated with RA in this population of From the Molecular Immunogenetics Unit and the Rheu-