Histone H4 proximal promoter mediates a complex transcriptional response during differentiation of 3T3L1 adipocytes
✍ Scribed by Dr. Anna L. Ramsey-Ewing; Rita Bortell; Gary S. Stein; Janet L. Stein
- Publisher
- John Wiley and Sons
- Year
- 1995
- Tongue
- English
- Weight
- 901 KB
- Volume
- 163
- Category
- Article
- ISSN
- 0021-9541
No coin nor oath required. For personal study only.
✦ Synopsis
We have investigated the promoter element(s) required by the cell cycle regulated FO108 human histone (\mathrm{H} 4) gene for control of gene expression during adipocyte proliferation and differentiation. Stable 3T3L1 cell lines were established that express fusion genes in which the histone (\mathrm{H} 4) promoter is joined to chloramphenicol acetyltransferase (cat) as a reporter gene. Expression of the H4CAT fusion genes was monitored in proliferating and confluent 3T3L1 preadipocytes and in differentiating 3T3L1 adipocytes. The results indicate that the (\mathrm{H} 4) cell cycle element (CCE), which mediates (S) phase-specific stimulation of (\mathrm{H} 4) gene transcription, is not required for transcriptional regulation during differentiation. Instead, a minimal H4 promoter (nucleotides -46 to -11 ) is sufficient to mediate the complex transcriptional response of (\mathrm{H} 4) gene expression observed during the process of adipocyte differentiation of 3T3L1 cells. In addition, the data suggest that down-regulation of histone gene expression during cellular differentiation may be mediated by passive inactivation of the promoter due to loss of positive regulatory factor(s). (@ 1995) Wiley-Liss, Inc.