## Abstract The effects of low level laser (LLL) irradiation on the proliferation of human buccal fibroblasts were studied. A standardized LLL set‐up was developed (812 nm, 4.5 ± 0.5 mW/cm2). Cultures in petridishes were divided into eight groups (1 group served as control). On day 6 after seeding,
Histomorphometric study of the healing of human oral mucosa after gingivoplasty and low-level laser therapy
✍ Scribed by Carla A. Damante; Sebastião L.A. Greghi; Adriana C.P. Sant'Ana; Euloir Passanezi; Rumio Taga
- Publisher
- John Wiley and Sons
- Year
- 2004
- Tongue
- English
- Weight
- 495 KB
- Volume
- 35
- Category
- Article
- ISSN
- 0196-8092
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✦ Synopsis
Abstract
Background and objectives
The aim of the present study was to analyze the effects of diode laser irradiation on the healing of human oral mucosa.
Materials and Methods
After gingivoplasty, the right hemi‐arch (test group) of 16 patients was irradiated with a diode laser. The left side (control group) was not irradiated. Incisional biopsies were performed on both sides at 7, 14, 21, and 60 days after surgery and morphometrically analyzed by light microscopy.
Results
Epithelium width ranged from 260.6 to 393.5 μm. Volume densities of basal (20.2%), prickle cell (55.6%), and cornified (24.2%) layers remained stable. The peak number of neutrophils were 6 cells/mm^2^ and the mononuclear cells were 44 cells/mm^2^. Collagen fibers (80%) and fibroblasts (14%) occupied the main volume of connective tissue. The one‐way ANOVA and the paired Student's t‐test were used for statistical analysis (P < 0.05).
Conclusion
Low‐level laser therapy did not accelerate the healing of oral mucosa after gingivoplasty. Lasers Surg. Med. 35:377–384, 2004. © 2004 Wiley‐Liss, Inc.
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