Histologic assessment of mesh fixation following laser-assisted tissue soldering in a lapine model
✍ Scribed by Raymond J. Lanzafame; Philip Brondon; Istvan Stadler; Dale P. DeVore; Robert Soltz; Barbara A. Soltz
- Book ID
- 102466017
- Publisher
- John Wiley and Sons
- Year
- 2005
- Tongue
- English
- Weight
- 232 KB
- Volume
- 37
- Category
- Article
- ISSN
- 0196-8092
No coin nor oath required. For personal study only.
✦ Synopsis
Objective:
Wound histology and mesh bioincorporation following intraperitoneal fixation using laser-assisted soldering was evaluated.
Methods:
2.8-3.2 kg nzw rabbits underwent laparotomy. controls had 2x2 cm segments of mersilene stapled to peritoneum. group 2 segments were affixed with 55% collagen solder onlay by fiber-coupled diode laser (1.43 +/- 10 micro, 2.5 w cw, 4 mm spot, 60 degrees c set temperature). group 4 had mersilene inlaid into melted solder. group 3 had solder-embedded vicryl mesh affixed. animals were euthanized at 0, 2, 4, 6 weeks. fixed sections were assessed for integrity, inflammation, and fibrosis using h & e, masson's trichrome and evans van gieson staining.
Results:
Histology demonstrated cell types, local mesh reaction, and progressive evidence of solder reabsorption mimicking normal healing and bioincorporation. mersilene groups demonstrated normal arrangement of collagen-rich layers around mesh.
Conclusion:
Collagen-based tissue soldering permits normal wound healing and may mitigate use of staples. further development of this strategy is warranted.