Histochemistry of single molecules: methods and protocols

✍ Scribed by Biggiogera, Marco;Pellicciari, Carlo

• Publisher: Humana Press : Springer
• Year: 2017
• Tongue: English
• Series: Methods in molecular biology (Humana Press) 1560
• Category: Library

✦ Synopsis

"This detailed volume explores numerous histochemical techniques through a series of lab-tested protocols for the detection of specific molecules or metabolic processes, both at light and electron microscopy. More in detail, the book is divided into six sections covering a variety of chemical targets. It begins with a section on vital histochemistry and continues with chapters on histochemistry as it relates to lectins, proteins, lipids, DNA and RNA, as well as plants. The volume also contains four overview chapters on vital histochemistry, lectin histochemistry, and DNA fluorochromes. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Histochemistry of Single Molecules: Methods and Protocols aims to effectively help scientists in very different research fields to elucidate biological issues though a unique approach to molecular biology in situ." -- OCLC.

✦ Table of Contents

Conté: Single cell cytochemistry illustrated by the demonstration of glucose-6-phosphate dehydrogenase deficiency in erythrocytes / Anna L. Peters and Cornelis J.F. van Noorden --
Autofluorescence spectroscopy for monitoring metabolism in animal cells and tissues / Anna C. Croce and Giovanni Bottiroli --
Enzyme-histochemistry technique for visualizing the dipeptidyl-peptidase IV (DPP-IV) activity in the liver biliary tree / Vittorio Bertone, Eleonora Tarantola, and Isabel Freitas --
Histochemical demonstration of tripeptidyl aminopeptidase I / Mashenka B. Dimitrova, Dimitrinka Y. Atanasova, and Nikolai E. Lazarov --
Enzyme histochemistry for functional histology in invertebrates / Francesca Cima --
Lectin histochemistry : historical perspectives, state of the art, and the future / Susan A. Brooks --
Isolation of viable glycosylation-specific cell populations for further in vitro or in vivo analysis using lectin-coated magnetic beads / Ellie-May Beaman, David R.F. Carter, and Susan A. Brooks --
Lectin histochemistry for metastasizing and non-metastasizing cancer cells / Gerrit Wolters-Eisfeld and Udo Schumacher --
Use of lectin histochemistry for detecting apoptotic cells in the seminiferous epithelium / Vicente Seco-Rovira, Ester Beltrán-Frutos, Jesús Hernández-Martínez, Concepción Ferrer, and Luis Miguel Pastor --
Heat-induced antigen retrieval in immunohistochemistry : mechanisms and applications / Shuji Yamashita and Osamu Katsumata --
Detecting neuronal differentiation markers in newborn cells of the adult brain / Sara Bonzano and Silvia De Marchis --
Characterizing satellite cells and myogenic progenitors during skeletal muscle regeneration / Nicolas A. Dumont and Michael A. Rudnicki --
Immunohistochemical detection of the autophagy markers LC3 and p62/SQSTM1 in formalin-fixed and paraffin-embedded tissue / Sabina Berezowska and José A. Galván --
Tissue fixation and processing for the histological identification of lipids / Víctor Carriel, Fernando Campos, José Aneiros-Fernández, and John A. Kiernan --
Staining methods for normal and regenerative myelin in the nervous system / Víctor Carriel, Antonio Campos, Miguel Alaminos, Stefania Raimondo, and Stefano Geuna --
Nile red staining of neutral lipids in yeast / Kerry Ann Rostron and Clare Louise Lawrence --
Staining of lipid droplets with monodansylpentane / Bo-Hua Chen, Huei-Jiun Yang, He-Yen Chou, Guang-Chao Chen, and Wei Yuan Yang --
Fluorochromes for DNA staining and quantitation / Giuliano Mazzini and Marco Danova --
Osmiumammine for staining DNA in electron microscopy / Irene Masiello and Marco Biggiogera --
DNA labeling at electron microscopy / Nicolas Thelen and Marc Thiry --
Visualizing RNA at electron microscopy by terbium citrate / Marco Biggiogera and Irene Masiello -Two-tailed comet assay (2T-Comet) : simultaneous detection of DNA single and double strand breaks / Elva I. Cortés-Gutiérrez, José Luis Fernández, Martha I. Dávila-Rodríguez, Carmen López-Fernández, and Jaime Gosálvez --
Detection of endogenous nuclear proteins in plant cells : localizing nuclear matrix constituent proteins (NMCPs), the plant analogs of lamins / Malgorzata Ciska and Susana Moreno Díaz de la Espina --
Histochemical analysis of plant secretory structures / Diego Demarco --
Histochemical technique for the detection of annonaceous acetogenins / Guillermo Laguna-Hernández, Alicia Enriqueta Brechú-Franco, Iván De la Cruz-Chacón, and Alma Rosa González-Esquinca.

✦ Subjects

Biologia molecular;Histoquímica;Llibres electrònics