Histochemical demonstration of sodium-dependent glutamate uptake in brain tissues by glutamate dehydrogenase reaction

✍ Scribed by G. Wolf; G. Schünzel; F. Rothe

• Book ID: 104676421
• Publisher: Springer-Verlag
• Year: 1986
• Tongue: English
• Weight: 656 KB
• Volume: 62
• Category: Article
• ISSN: 0014-4819
• DOI: 10.1007/bf00236047

✦ Synopsis

Using a special tetrazolium salt technique, a striking correlation was observed between Na+ concentration of the incubation medium and the formation of formazan catalyzed by glutamate dehydrogenase (GDH) in glutamatergic neuropil areas of the hippocampal formation, cerebellum, and other brain regions. Na+ concentrations of 130-150 mmol/l caused maximal formazan production. The GDH catalyzed sodium-dependent increase in formazan production is suggested to be a consequence of the sodium dependence of glutamate uptake in glutamatergic brain structures supplying the enzyme with substrate.