Histidine-rich peptide: evidence for a single zinc-binding site on H5WYG peptide that promotes membrane fusion at neutral pH
✍ Scribed by Corinne Buré; Régine Maget; Agnès F. Delmas; Chantal Pichon; Patrick Midoux
- Book ID
- 102377161
- Publisher
- John Wiley and Sons
- Year
- 2009
- Tongue
- English
- Weight
- 418 KB
- Volume
- 44
- Category
- Article
- ISSN
- 1076-5174
- DOI
- 10.1002/jms.1473
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✦ Synopsis
Abstract
The histidine‐rich peptide H5WYG (GLFHAIAHFIHGGWHGLIHGWYG) was found to induce membrane fusion at physiologic pH in the presence of zinc chloride. In this study, we examined the ion selectivity of the interaction of Zn^2+^ with H5WYG. This investigation was conducted by using adsorption at air/water interface and mass spectrometry. We found that a peptide–metal complex is formed with Zn^2+^ ions. Electrospray ionisation‐mass spectrometry (ESI‐MS) reveals that the [H5WYG + Zn + 2H]^4+^, [H5WYG + Zn + H]^3+^ and [H5WYG + Zn]^2+^ ions, appearing by increasing the amount of Zn^2+^ equivalent, correspond to a monomolecular H5WYG − Zn^2+^ complex. Tandem mass spectrometry (MS/MS) provides evidence for the binding of the single Zn^2+^ ion to the H^11^ and H^19^ and probably H^15^ residues. Copyright © 2008 John Wiley & Sons, Ltd.