Histamine-induced cyclic AMP accumulation in type-1 and type-2 astrocytes in primary culture
β Scribed by Akiharu Kubo; Hiroyuki Fukui; Naoyuki Inagaki; Akira Kanamura; Hiroshi Wada
- Book ID
- 113398235
- Publisher
- Elsevier Science
- Year
- 1991
- Tongue
- English
- Weight
- 456 KB
- Volume
- 208
- Category
- Article
- ISSN
- 0922-4106
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The regulation of intracellular cyclic AMP (CAMP) formation by adenosine (Ado) and its analogues has been examined in primary cultures of rat-brain astrocytes and neurons. In the presence of the phosphodiesterase inhibitor, Ro 20-1724, basal levels of cAMP ranged from 40-120 pmoVmg protein in both c
Cultured astrocytes express bradykinin (BK) receptors, which are coupled to phospholipase C (PLC) through G-protein to mediate phosphoinositide (PI) hydrolysis. The regulation of this BK receptor-G protein-PLC pathway by CAMP and endothelin-1 (ET-1) was explored by short-term (20 min) and long-term