Histamine effects on the 5-HT1c receptor expressed in Xenopus oocytes

✍ Scribed by S. Shichijo; D. G. Payan; G. Harrowe; Dr.; M.D. M. Mitsuhashi

• Publisher: John Wiley and Sons
• Year: 1991
• Tongue: English
• Weight: 478 KB
• Volume: 30
• Category: Article
• ISSN: 0360-4012
• DOI: 10.1002/jnr.490300206

✦ Synopsis

Abstract

To analyze the cross‐reactivity between serotonin (5‐HT) and histamine, the in vitro transcribed RNA for the 5‐HT~1c~ receptor was functionally expressed in Xenopus oocytes. 5‐HT significantly increased ^45^Ca^2+^ efflux in RNA‐injected oocytes, but not in uninjected and water‐injected control oocytes. Furthermore, histamine and the H~1~ receptor agonists, but not the H~2~ and H~3~ agonists, significantly induced ^45^Ca^2+^ efflux in 5‐HT1c receptor RNA‐injected oocytes, but not in uninjected and water‐injected oocytes. However, the H~1~, H~2~, and H~3~ antagonists failed to inhibit histamine‐induced ^45^Ca^2+^ efflux at 10^−6^M. This finding suggests that the 5‐HT~1c~ receptor can be activated by both 5‐HT and histamine, although the action of histamine is different from classic histamine pharmacology.