Highly Sensitive Detection of Hepatitis B Virus Surface Antigen by Use of a Semiautomated Immune Complex Transfer Chemiluminescence Enzyme Immunoassay
โ Scribed by Takeda, K.; Maruki, M.; Yamagaito, T.; Muramatsu, M.; Sakai, Y.; Tobimatsu, H.; Kobayashi, H.; Mizuno, Y.; Hamaguchi, Y.
- Book ID
- 126986289
- Publisher
- American Society for Microbiology
- Year
- 2013
- Tongue
- English
- Weight
- 642 KB
- Volume
- 51
- Category
- Article
- ISSN
- 0095-1137
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## Abstract We developed a highly sensitive enzyme immunoassay (EIA), the pโAP/HHTIO method, that detects serum hepatitis B surface antigen (HBsAg) by measuring stabilized nitroxide radicals using a novel electron spin resonance technique [Matsuo et al. (1998) Free Radic Biol Med 25:929โ935]. To de
For diagnosis of HIV-1 infection, attempts were made to detect anti-HIV-1 IgG in urine by sensitive enzyme immunoassay (immune complex transfer enzyme immunoassay) US- ing recombinant reverse transcriptase (RT) and p17 as antigens. Anti-HIV-1 IgG in urine was reacted simultaneously with 2,4-dinitrop