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Highly sensitive colorimetric method for the detection of Salmonella spp. in environmental and clinical samples

✍ Scribed by D. Prasad; A.S. Vidyarthi

Publisher
Elsevier
Year
2009
Tongue
English
Weight
78 KB
Volume
25
Category
Article
ISSN
1871-6784

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✦ Synopsis

Prodigiosin and pyoverdine pigment produced by Serritia marcescens and Pseudomonas fluorescens have been purified and characterized by UV-vis spectra. The interaction of bioactive pigments with calf thymus DNA (ct-DNA) has been explored by UV-vis and viscosity measurements. The results suggest that these prodiogiosin and pyoverdine can bind to DNA by two different modes. Prodiogiosin intercalate between the base pair like ethidium bromide (EB) and pyoverdine, which bind in the minor groove of DNA, from the experimental evidences.

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