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Highly selective biosensing of lactate at lactate oxidase containing rhodium-dispersed carbon paste electrodes

✍ Scribed by Joseph Wang; Qiang Chen; Maria Pedrero

Publisher
Elsevier Science
Year
1995
Tongue
English
Weight
500 KB
Volume
304
Category
Article
ISSN
0003-2670

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✦ Synopsis

A remarkably selective lactate biosensor, based on the immobilization of lactate oxidase within a rhodium-dispersed carbon paste is described. The effective catalytic action of the rhodium microparticles towards the generated hydrogen peroxide or consumed oxygen allows tuning of the operating potential to a region where unwanted reactions are prevented. Contributions from all major easily oxidizable substances are eliminated, without the need for membrane barriers or artificial electron mediators. The detection limit is 1.5 X lo-' M and the response time is 8 s. A fast and sensitive flow injection operation is illustrated.

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## Abstract This work reports the advantages of carbon paste electrodes modified with electrogenerated magnetite nanoparticles. The nanoparticles present catalytic activity towards hydrogen peroxide reduction. The incorporation of glucose oxidase (GOx) and magnetite in a carbon paste matrix have ma