Highly Efficient Single Molecule Detection in Different Micro and submicrometer Channels with cw-excitation

Single-molecule detection with total int

Single-molecule detection with total internal reflection excitation: Comparing signal-to-background and total signals in different geometries

✍ W. Patrick Ambrose; Peter M. Goodwin; John P. Nolan 📂 Article 📅 1999 🏛 John Wiley and Sons 🌐 English ⚖ 278 KB

Excitation of fluorescence with total internal reflection (TIR) excitation yields very low background scattered light and good signal-to-background contrast. The background and its associated noise can be made low enough to detect single fluorescent molecules under ambient conditions. In this paper,