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Highly Efficient Biosynthesis of the Oligosaccharide Moiety of the GD3 Ganglioside by Using Metabolically Engineered Escherichia coli

✍ Scribed by Tatiana Antoine; Alain Heyraud; Claude Bosso; Eric Samain

Publisher: John Wiley and Sons
Year: 2005
Tongue: English
Weight: 107 KB
Volume: 44
Category: Article
ISSN: 0044-8249
DOI: 10.1002/anie.200461507

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## Abstract __Two metabolically engineered__ Escherichia coli __strains have been constructed to produce the carbohydrate moieties of gangliosides GM2 (GalNAc__β__‐4(NeuAc__α__‐3)Gal__β__‐4Glc; Gal=galactose, Glc=glucose, Ac=acetyl) and GM1 (Gal__β__‐3GalNAc__β__‐4(NeuAc__α__‐3)Gal__β__‐4Glc. The G