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High-Yield Formation of Arbutin from Hydroquinone by Cell-Suspension Cultures of Rauwolfia serpentina

✍ Scribed by Ralf Lutterbach; Joachim Stöckigt

Publisher: John Wiley and Sons
Year: 1992
Tongue: German
Weight: 226 KB
Volume: 75
Category: Article
ISSN: 0018-019X
DOI: 10.1002/hlca.19920750625

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✦ Synopsis

Abstract

High‐density cell‐suspension cultures of Rauwolfia serpentina cultivated in a nutrition medium optimized for the production of the glucoalkaloid raucaffricine synthesize hydroquinone glycosides from continuously added hydroquinone with a total yield of 23.87 g/1 (18 g/1 of arbutin and 5.87 g/1 of a hydroquinone diglycoside) in 7 days. This arbutin production is by far the highest formation of a natural product by plant‐cell‐culture systems reported to date.

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Abbreoiutions: ADP, adenosin 5'-diposphate; CDP, cytidine 5'-diphosphate; GDP, guanosine 5'-diphosphate; TDP, thymidine 5'-diphosphate. UDPG ( = UDP-Glc), uridine 5