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High-yield cell-free protein synthesis for site-specific incorporation of unnatural amino acids at two sites

✍ Scribed by Kiyoshi Ozawa; Karin V. Loscha; Kekini V. Kuppan; Choy Theng Loh; Nicholas E. Dixon; Gottfried Otting

Book ID
116302933
Publisher
Elsevier Science
Year
2012
Tongue
English
Weight
399 KB
Volume
418
Category
Article
ISSN
0006-291X

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High-level cell-free synthesis yields of
High-level cell-free synthesis yields of proteins containing site-specific non-natural amino acids
✍ Aaron R. Goerke; James R. Swartz πŸ“‚ Article πŸ“… 2009 πŸ› John Wiley and Sons 🌐 English βš– 500 KB πŸ‘ 2 views

## Abstract We describe an __E. coli__‐based cell‐free system for the production of proteins with a non‐natural amino acid (nnAA) incorporated site‐specifically (modified protein). The mutant __Methanococcus jannaschii__ tyrosyl‐tRNA synthetase (mTyrRS) and tRNA^Tyr^ pair were used as orthogonal el