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High yield and secretion of recombinant human apolipoprotein AI in Pichia pastoris

✍ Scribed by Mei-qing Feng; Qin-sheng Cai; Da-xin Song; Ji-bin Dong; Pei Zhou


Book ID
116828297
Publisher
Elsevier Science
Year
2006
Tongue
English
Weight
459 KB
Volume
46
Category
Article
ISSN
1046-5928

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Recombinant non-hydroxylated gelatins based on mouse type I and rat type III collagen sequences were secreted from the methylotrophic yeast Pichia pastoris, using the Saccharomyces cerevisiae alpha-mating factor prepro signal. Proteolytic degradation could be minimized to a large extent by performin