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High Uniformity of plants regenerated from cytogenetically variable embryogenic suspension cultures of poinsettia (Euphorbia pulcherrimaWilld. ex Klotzsch)

✍ Scribed by Thomas Geier; Andrea Beck; Walter Preil


Publisher
Springer
Year
1992
Tongue
English
Weight
463 KB
Volume
11
Category
Article
ISSN
0721-7714

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✦ Synopsis


Shoot tip explants, callus and embryogenic cell suspensions of Euphorbia pulcherrima have been examined for quantitative variation in nuclear DNA content by means of cytophotometry. Increasing instability was found in calli and cell suspensions from Erlenmeyer flask and bioreactor culture. Nuclear DNA content ranged from 2C up to 32 C. Plants regenerated from embryogenic cell suspensions, however, were highly uniform with regard to phenotype and ploidy level indicating strongly impaired embryogenic potential of polyploid, aneuploid or other genetically altered cells.