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High-throughput RNAi screening to dissect cellular pathways: A how-to guide

โœ Scribed by Christina Falschlehner; Sandra Steinbrink; Gerrit Erdmann; Michael Boutros


Book ID
112012085
Publisher
John Wiley and Sons
Year
2010
Tongue
English
Weight
378 KB
Volume
5
Category
Article
ISSN
1860-6768

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Four-enzyme section of the shikimate pathway (Aro B, D, E, and K) of Streptococcus pneumoniae has been studied. Kinetic properties of the individual enzymes and three-and four-enzyme linked reactions have been characterized in vitro. On the basis of the data measured in spectrophotometric and LC-MS