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High-throughput production of human proteins for crystallization: The SGC experience

✍ Scribed by Pavel Savitsky; James Bray; Christopher D.O. Cooper; Brian D. Marsden; Pravin Mahajan; Nicola A. Burgess-Brown; Opher Gileadi


Book ID
116697104
Publisher
Elsevier Science
Year
2010
Tongue
English
Weight
752 KB
Volume
172
Category
Article
ISSN
1047-8477

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## Abstract The baculovirus expression system is one of the most popular methods used for the production of recombinant proteins but has several complex steps which have proved inherently difficult to adapt to a multi‐parallel process. We have developed a bacmid vector that does not require any for