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High-throughput mapping of origins of replication in human cells

✍ Scribed by Lucas, Isabelle; Palakodeti, Aparna; Jiang, Yanwen; Young, David J; Jiang, Nan; Fernald, Anthony A; Le Beau, Michelle M

Book ID: 110033370
Publisher: Nature Publishing Group
Year: 2007
Tongue: English
Weight: 311 KB
Volume: 8
Category: Article
ISSN: 1469-221X
DOI: 10.1038/sj.embor.7401026

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## Abstract A quantitative PCR method was used to map a functional origin of DNA replication in the hypoxanthine‐guanine phosphoribosyltransferase (HPRT) gene in normal human fibroblasts. This PCR method measures the abundance of specific sequences in short fragments of newly replicated DNA from lo