✦ LIBER ✦

High-throughput genotyping of repeat polymorphism in the regulatory region of serotonin transporter gene by gel microchip electrophoresis

✍ Scribed by Zsofia Nemoda; Zsolt Ronai; Anna Szekely; Erika Kovacs; Sarah Shandrick; András Guttman; Maria Sasvari-Szekely

Publisher: John Wiley and Sons
Year: 2001
Tongue: English
Weight: 564 KB
Volume: 22
Category: Article
ISSN: 0173-0835
DOI: 10.1002/1522-2683(200110)22:18<4008::aid-elps4008>3.0.co;2-#

No coin nor oath required. For personal study only.

✦ Synopsis

High-throughput genotyping of repeat polymorphism in the regulatory region of serotonin transporter gene by gel microchip electrophoresis

Large-scale genotyping of the repeat polymorphism in the regulatory region of the serotonin transporter gene (5-HTTLPR) was attempted by polymerase chain reaction (PCR) amplification followed by gel microchip electrophoresis analysis. The multilane (96) format of the gel microchip system allowed parallel separation of a large number of samples. The separation and visualization of the PCR amplicons from either the 5-HTTLPR short allele (number of repeats are 14) or the 5-HTTLPR long form (16 repeats) was completed in a few minutes. Genotyping of healthy Caucasian individuals showed that the short allele had a somewhat lower frequency (0.42) than the long form (0.58), and the genotype frequencies fulfilled the criteria of the Hardy-Weinberg equilibrium (w = 0.012, p = 0.994). Based on these results, gel microchip electrophoresis system proved to be a powerful tool for high throughput genotyping of repeat polymorphism.

📜 SIMILAR VOLUMES

Possible association of fibromyalgia wit

Possible association of fibromyalgia with a polymorphism in the serotonin transporter gene regulatory region

✍ Martin Offenbaecher; Brigitta Bondy; Silvia De Jonge; Karin Glatzeder; Marc Krüg 📂 Article 📅 1999 🏛 John Wiley and Sons 🌐 English ⚖ 141 KB 👁 2 views

Genotyping the –6A/G functional polymorp

Genotyping the –6A/G functional polymorphism in the core promoter region of angiotensinogen gene by microchip electrophoresis

✍ Jianhua Qin; Zhaoyi Liu; Dapeng Wu; Ning Zhu; Xiaomian Zhou; Yingsing Fung; Bing 📂 Article 📅 2005 🏛 John Wiley and Sons 🌐 English ⚖ 246 KB

Analysis of a novel functional polymorph

Analysis of a novel functional polymorphism within the promoter region of the serotonin transporter gene (5-HTT) in Brazilian patients affected by bipolar disorder and schizophrenia

✍ de Oliveira, Jo�o R. Mendes; Otto, Paulo A.; Vallada, Homero; Lauriano, Val�ria; 📂 Article 📅 1998 🏛 John Wiley and Sons 🌐 English ⚖ 11 KB 👁 2 views

It has been suggested that the serotonin transporter (5-hydroxytryptamine-transporter or 5-HTT) may be involved in the pathogenesis of affective disorders. Recently, Collier et al. (1996) found that the frequency of the low-activity short variant (s) of the 5-HTT-linked polymorphic region (5-HTTLPR)