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High-throughput generation and engineering of recombinant human antibodies

✍ Scribed by Barbara Krebs; Robert Rauchenberger; Silke Reiffert; Christine Rothe; Michael Tesar; Elisabeth Thomassen; Manqiu Cao; Torsten Dreier; David Fischer; Adolf Höß; Landon Inge; Achim Knappik; Matthias Marget; Peter Pack; Xian-Qin Meng; Robert Schier; Peter Söhlemann; Jill Winter; Joachim Wölle; Titus Kretzschmar

Book ID
114050791
Publisher
Elsevier Science
Year
2001
Tongue
English
Weight
486 KB
Volume
254
Category
Article
ISSN
0022-1759

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We describe a method for high-throughput, parallel purification of secreted proteins to analyse large numbers of protein samples in cell-based assays for the discovery of protein therapeutics. The procedure is generic and capable of 96 parallel purifications and compatible, in both yield and purity,