✦ LIBER ✦

High-Throughput Fluorogenic Assay for Determination of Botulinum Type B Neurotoxin Protease Activity

✍ Scribed by Christine Anne; Fabrice Cornille; Christine Lenoir; Bernard P. Roques

Publisher: Elsevier Science
Year: 2001
Tongue: English
Weight: 107 KB
Volume: 291
Category: Article
ISSN: 0003-2697
DOI: 10.1006/abio.2001.5028

No coin nor oath required. For personal study only.

📜 SIMILAR VOLUMES

High-Throughput Assays for Botulinum Neu

High-Throughput Assays for Botulinum Neurotoxin Proteolytic Activity: Serotypes A, B, D, and F

✍ James J. Schmidt; Robert G. Stafford; Charles B. Millard 📂 Article 📅 2001 🏛 Elsevier Science 🌐 English ⚖ 99 KB

Botulinum neurotoxins (BoNT) are zinc metalloproteases that cleave and inactivate cellular proteins essential for neurotransmitter release. Because the paralytic effect of BoNT is a consequence of its enzymatic activity, selective inhibitors may be useful as drugs or as tools for further research. T

An Assay for High-Sensitivity Detection

An Assay for High-Sensitivity Detection of Thrombin Activity and Determination of Proteases Activating or Inactivating Protease-Activated Receptors

✍ Ludger M. Altrogge; Denis Monard 📂 Article 📅 2000 🏛 Elsevier Science 🌐 English ⚖ 336 KB

This paper describes the development of galactosidase protease-activated receptor (GPAR) as a recombinant protein obtained by fusion of ␤-galactosidase, the extracellular domains of protease-activated receptors (PARs), and a biotin acceptor domain. Used as an immobilized substrate, this protein allo

A High-Throughput Microtiter Plate-Based

A High-Throughput Microtiter Plate-Based Calcium Assay for the Study of Protease-Activated Receptor 2 Activation

✍ Susan J. Hawthorne; Marion Macey; Gareth Howells; Brian Walker 📂 Article 📅 2001 🏛 Elsevier Science 🌐 English ⚖ 44 KB

A Colorimetric Method for the Determinat

A Colorimetric Method for the Determination of Lipoxygenase Activity Suitable for Use in a High Throughput Assay Format

✍ Neil B. Waslidge; David J. Hayes 📂 Article 📅 1995 🏛 Elsevier Science 🌐 English ⚖ 87 KB

throughput assay. Therefore, there is a need to develop A rapid and sensitive method for measuring the aca method that is rapid, reproducible, and suitable for tivity of lipoxygenase is described. The assay is based adaptation to a high throughput assay format. Here on the principle that under acidi