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High-throughput automated refolding screening of inclusion bodies

✍ Scribed by Renaud Vincentelli; Stéphane Canaan; Valérie Campanacci; Christel Valencia; Damien Maurin; Frédéric Frassinetti; Loréna Scappucini-Calvo; Yves Bourne; Christian Cambillau; Christophe Bignon

Book ID
119824532
Publisher
Cold Spring Harbor Laboratory Press
Year
2009
Tongue
English
Weight
179 KB
Volume
13
Category
Article
ISSN
0961-8368

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## Abstract We established a 96‐well‐plate‐based refolding screening system using zeolite. In this system, protein denatured and solubilized with 6 M guanidine hydrochloride is adsorbed onto zeolite placed in a 96‐well plate. The refolding conditions can be tested by incubating the samples with ref