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High-speed DNA sequencing in ultrathin slab gels

✍ Scribed by Thomas D Yager; James M Dunn; John K Stevens

Book ID
104362081
Publisher
Elsevier Science
Year
1997
Tongue
English
Weight
814 KB
Volume
8
Category
Article
ISSN
0958-1669

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✦ Synopsis

There have been many recent theoretical and technical advances in the sequencing of DNA in ultrathin slab gels. These include recent experimental progress in four areas: DNA polymerases; DNA template preparation; the separation and detection of DNA bands; and base-calling algorithms. The Zimm-Lumpkin theory of electrophoresis provides a new and improved framework for understanding the operation of sequencing gels.

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## Abstract We assessed the feasibility of high‐speed DNA sequencing by tube‐based capillary electrophoresis (TCE) with electrokinetic sample injections. We developed a water‐circulated TCE system to control the capillary temperature precisely. Using this system and a ready‐made sieving matrix at 5