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High speed DNA sequencing by capillary electrophoresis

✍ Scribed by Luckey, J. A.; Drossman, H.; Kostichka, A. J.; Mead, D. A.; D'Cunha, J.; Norris, T. B.; Smith, L. M.


Book ID
121530885
Publisher
Oxford University Press
Year
1990
Tongue
English
Weight
732 KB
Volume
18
Category
Article
ISSN
0305-1048

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## Abstract We assessed the feasibility of high‐speed DNA sequencing by tube‐based capillary electrophoresis (TCE) with electrokinetic sample injections. We developed a water‐circulated TCE system to control the capillary temperature precisely. Using this system and a ready‐made sieving matrix at 5

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## Contents Capillary electrophoresis has been under development for DNA sequencing since 1990. This development has traveled down two parallel tracks. The first track studied the details of DNA separation by gel electrophoresis. Early work stressed rapid separations at high electric fields, which