High speed DNA sequencing by capillary electrophoresis
β Scribed by Luckey, J. A.; Drossman, H.; Kostichka, A. J.; Mead, D. A.; D'Cunha, J.; Norris, T. B.; Smith, L. M.
- Book ID
- 121530885
- Publisher
- Oxford University Press
- Year
- 1990
- Tongue
- English
- Weight
- 732 KB
- Volume
- 18
- Category
- Article
- ISSN
- 0305-1048
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## Abstract We assessed the feasibility of highβspeed DNA sequencing by tubeβbased capillary electrophoresis (TCE) with electrokinetic sample injections. We developed a waterβcirculated TCE system to control the capillary temperature precisely. Using this system and a readyβmade sieving matrix at 5
## Contents Capillary electrophoresis has been under development for DNA sequencing since 1990. This development has traveled down two parallel tracks. The first track studied the details of DNA separation by gel electrophoresis. Early work stressed rapid separations at high electric fields, which