High-speed Analysis of Catecholamines in Plasma by Medium-Pressure μ-LC with an Amperometric Detector
✍ Scribed by V. F. Ruban; S. N. Petunin; N. C. Merkulova
- Publisher
- John Wiley and Sons
- Year
- 1996
- Tongue
- English
- Weight
- 441 KB
- Volume
- 10
- Category
- Article
- ISSN
- 0269-3879
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✦ Synopsis
A high-speed highly sensitive and reproducible method for the determination of catecholamines in small plasma volumes has been developed. Catecholamines were separated on a short capillary fused-silica column (0.32 mm i.d.) packed with a reverse-phase sorbent with a particle size of 10 pm. The time of a single chromatographic analysis was less than 6 min, and the operating pressure in the system was not higher than 5.0 MPa. The required volume of blood plasma was 0.5 mL. When a 10 pL sample previously concentrated on alumina was injected into the column, the limits of amperometric detection for noradrenaline (NA), adrenaline (A), 3,4-dihydroxybenzylamine (DHBA), and dopamine @A) were 3.9, 2.6, 1.8 and 5.6 n&, respectively. The average variation coefficient was 17.4%.